The Human (MARK4) Map, Microtubule Affinity-Regulating Kinase 4 ELISA Kit measures Map,Microtubule Affinity-Regulating Kinase 4 in samples. The plate has been pre-coated with Human MARK4 antibody. MARK4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MARK4 Antibody is added and binds to MARK4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MARK4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MARK4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasSignal Transduction, Enzyme & Kinase
BackgroundSerine/threonine-protein kinase (PubMed: 15009667, PubMed: 14594945, PubMed: 23666762, PubMed: 23184942). Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed: 14594945, PubMed: 23666762). Also phosphorylates the microtubule-associated proteins MAP2 and MAP4 (PubMed: 14594945). Involved in regulation of the microtubule network, causing reorganization of microtubules into bundles (PubMed: 14594945, PubMed: 25123532). Required for the initiation of axoneme extension during cilium assembly (PubMed: 23400999). Regulates the centrosomal location of ODF2 and phosphorylates ODF2 in vitro (PubMed: 23400999). Plays a role in cell cycle progression, specifically in the G1/S checkpoint (PubMed: 25123532). Reduces neuronal cell survival (PubMed: 15009667). Plays a role in energy homeostasis by regulating satiety and metabolic rate (By similarity). Promotes adipogenesis by activating JNK1 and inhibiting the p38MAPK pathway, and triggers apoptosis by activating the JNK1 pathway (By similarity). Phosphorylates mTORC1 complex member RPTOR and acts as a negative regulator of the mTORC1 complex, probably due to disruption of the interaction between phosphorylated RPTOR and the RRAGA/RRAGC heterodimer which is required for mTORC1 activation (PubMed: 23184942). Source: UniProt Consortium (2025)
