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Human Mhc Class I Polypeptide-Related Sequence B (MICB) ELISA Kit

Human Mhc Class I Polypeptide-Related Sequence B (MICB) ELISA Kit

This ELISA kit is designed to detect Human Mhc Class I Polypeptide-Related Sequence B (Human MICB). The assay plate has been pre-coated with mouse anti-Human MICB monoclonal antibody. When the sample containing MICB is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human MICB Antibody is added to the wells and binds to MICB in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human MICB present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE381
Regular price $624.00 USD
Regular price $480.00 USD Sale price $624.00 USD
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Product Details

Species Reactivity Human
Sensitivity 24.49 pg/mL
Detection Range 78.12-5000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Immunology
Background MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without the capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte MHC class I polypeptide-related sequence B
Regulatory Status For Research Use Only
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