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Human MLKL (Mixed Lineage Kinase Domain-like Protein) ELISA Kit
Human MLKL (Mixed Lineage Kinase Domain-like Protein) ELISA Kit
The Human (MLKL) Mixed Lineage Kinase Domain-like Protein ELISA Kit measures Mixed Lineage Kinase Domain-Like Protein in samples. The plate has been pre-coated with Human MLKL antibody. MLKL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MLKL Antibody is added and binds to MLKL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MLKL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MLKL. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1875Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
11.18 ng/L
Detection Range
20-4500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Enzyme & Kinase
Background
Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 24316671). Does not have protein kinase activity (PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 24316671). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 24316671). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following activation by ZBP1, MLKL is phosphorylated by RIPK3 in the nucleus, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (By similarity). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (PubMed: 29883610). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Mixed Lineage Kinase Domain-Like Protein
Regulatory Status
For Research Use Only
