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Human MMP-25 (Matrix Metalloproteinase 25) ELISA Kit
Human MMP-25 (Matrix Metalloproteinase 25) ELISA Kit
The Human (MMP-25) Matrix Metalloproteinase 25 ELISA Kit measures Matrix Metalloproteinase 25 in samples. The plate has been pre-coated with Human MMP-25 antibody. MMP-25 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MMP-25 Antibody is added and binds to MMP-25 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MMP-25 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MMP-25. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4627Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.11 ng/mL
Detection Range
0.2-60 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
Matrix metalloproteinase-25 is an enzyme that in humans is encoded by the MMP25 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Matrix Metalloproteinase 25
Regulatory Status
For Research Use Only
