1
/
of
1
Human MPHOSPH8 (M-phase Phosphoprotein 8) ELISA Kit
Human MPHOSPH8 (M-phase Phosphoprotein 8) ELISA Kit
The Human (MPHOSPH8) M-phase Phosphoprotein 8 ELISA Kit measures M-Phase Phosphoprotein 8 in samples. The plate has been pre-coated with Human MPHOSPH8 antibody. MPHOSPH8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MPHOSPH8 Antibody is added and binds to MPHOSPH8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MPHOSPH8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MPHOSPH8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2915Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Human
Sensitivity
1.17 ng/L
Detection Range
2-700 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Heterochromatin component that specifically recognizes and binds methylated 'Lys-9' of histone H3 (H3K9me) and promotes recruitment of proteins that mediate epigenetic repression (PubMed: 20871592, PubMed: 26022416). Mediates recruitment of the HUSH complex to H3K9me3 sites: the HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed: 26022416, PubMed: 28581500). Binds H3K9me and promotes DNA methylation by recruiting DNMT3A to target CpG sites; these can be situated within the coding region of the gene (PubMed: 20871592). Mediates down-regulation of CDH1 expression (PubMed: 20871592). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed: 29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed: 30487602). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
M-Phase Phosphoprotein 8
Regulatory Status
For Research Use Only
