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Human MTHFR (Methylenetetrahydrofolate Reductase) ELISA Kit

Human MTHFR (Methylenetetrahydrofolate Reductase) ELISA Kit

The Human (MTHFR) Methylenetetrahydrofolate Reductase ELISA Kit measures Methylenetetrahydrofolate Reductase in samples. The plate has been pre-coated with Human MTHFR antibody. MTHFR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MTHFR Antibody is added and binds to MTHFR in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MTHFR antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MTHFR. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E4352hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.093 ng/mL
Detection Range 0.2-60 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Methylenetetrahydrofolate Reductase
Regulatory Status For Research Use Only
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