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Human MTR (Methionine Synthase) ELISA Kit
Human MTR (Methionine Synthase) ELISA Kit
The Human (MTR) Methionine Synthase ELISA Kit measures Methionine Synthase in samples. The plate has been pre-coated with Human MTR antibody. MTR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MTR Antibody is added and binds to MTR in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MTR antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MTR. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3942Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.027 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Metabolism
Background
Catalyzes the transfer of a methyl group from methylcob(III)alamin (MeCbl) to homocysteine, yielding enzyme-bound cob(I)alamin and methionine in the cytosol (PubMed: 27771510). MeCbl is an active form of cobalamin (vitamin B12) used as a cofactor for methionine biosynthesis. Cob(I)alamin form is regenerated to MeCbl by a transfer of a methyl group from 5-methyltetrahydrofolate (PubMed: 27771510). The processing of cobalamin in the cytosol occurs in a multiprotein complex composed of at least MMACHC, MMADHC, MTRR (methionine Synthase reductase) and MTR which may contribute to shuttle safely and efficiently cobalamin towards MTR in order to produce methionine (PubMed: 27771510). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Methionine Synthase
Regulatory Status
For Research Use Only
