The Human (NDUFS1) Nadh-ubiquinone Oxidoreductase 75 kDa Subunit ELISA Kit measures NADH-ubiquinone oxidoreductase 75 kDa subunit in samples. The plate has been pre-coated with Human NDUFS1 antibody. NDUFS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NDUFS1 Antibody is added and binds to NDUFS1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NDUFS1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NDUFS1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasTags and Cell Markers, Neuroscience, Cancer, Metabolism
BackgroundNADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1) is an enzyme that in humans is encoded by the NDUFS1 gene. The encoded protein, NDUFS1, is the largest subunit of complex I, located on the inner mitochondrial membrane, and is important for mitochondrial oxidative phosphorylation. Mutations in this gene are associated with complex I deficiency. The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Source: UniProt Consortium (2025)
