The Human (NDUFS7) Nadh Dehydrogenase, Ubiquinone, Iron-sulfur Protein 7 ELISA Kit measures NADH dehydrogenase [ubiquinone] iron-sulfur protein 7 in samples. The plate has been pre-coated with Human NDUFS7 antibody. NDUFS7 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NDUFS7 Antibody is added and binds to NDUFS7 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NDUFS7 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NDUFS7. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasTags and Cell Markers, Signal Transduction, Neuroscience, Metabolism
BackgroundNADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial, also knowns as NADH-ubiquinone oxidoreductase 20 kDa subunit, Complex I-20kD (CI-20kD), or PSST subunit is an enzyme that in humans is encoded by the NDUFS7 gene. The PSST subunit encoded by the NDUSF7 gene is one of over 40 subunits involved in the transfer of electrons from NADH to ubiquinone. Specifically, it is thought that the PSST subunit directly couples electron transfer between the iron-sulfur cluster N2 and ubiquinone, along with ubiquinone-binding ND1.Functional evidence for the importance of PSST has been garnered from mutational studies in the obligate aerobic yeast, Yarrow lipolytic, which elucidated a central role in proton translocation that was reduced in mutant forms of the subunit. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteNADH dehydrogenase [ubiquinone] iron-sulfur protein 7
