The Human (NDUFS8) Nadh Dehydrogenase, Ubiquinone, Iron-sulfur Protein 8 ELISA Kit measures NADH dehydrogenase [ubiquinone] iron-sulfur protein 8 in samples. The plate has been pre-coated with Human NDUFS8 antibody. NDUFS8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NDUFS8 Antibody is added and binds to NDUFS8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NDUFS8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NDUFS8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasTags and Cell Markers, Signal Transduction, Cancer, Metabolism
BackgroundNADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial also known as NADH-ubiquinone oxidoreductase 23 kDa subunit, Complex I-23kD (CI-23kD), or TYKY subunit is an enzyme that in humans is encoded by the NDUFS8 gene. This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteNADH dehydrogenase [ubiquinone] iron-sulfur protein 8
