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Human NE (Neutrophil Elastase) ELISA Kit
Human NE (Neutrophil Elastase) ELISA Kit
The Human (NE) Neutrophil Elastase ELISA Kit measures Neutrophil Elastase in samples. The plate has been pre-coated with Human ELANE antibody. ELANE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ELANE Antibody is added and binds to ELANE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ELANE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ELANE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0890Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.251 ng/mL
Detection Range
0.5-100 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed: 15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed: 10947984). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Neutrophil Elastase
Regulatory Status
For Research Use Only
