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Human NEFH (Neurofilament Heavy Polypeptide) ELISA Kit
Human NEFH (Neurofilament Heavy Polypeptide) ELISA Kit
The Human (NEFH) Neurofilament Heavy Polypeptide ELISA Kit measures Neurofilament Heavy Polypeptide in samples. The plate has been pre-coated with Human NEFH antibody. NEFH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NEFH Antibody is added and binds to NEFH in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NEFH antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NEFH. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3136Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.27 ng/mL
Detection Range
0.5-150 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Neuroscience
Background
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Neurofilament Heavy Polypeptide
Regulatory Status
For Research Use Only
