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Human NEFL (Neurofilament, Light Polypeptide) ELISA Kit
Human NEFL (Neurofilament, Light Polypeptide) ELISA Kit
The Human (NEFL) Neurofilament, Light Polypeptide ELISA Kit measures Neurofilament, Light Polypeptide in samples. The plate has been pre-coated with Human NEFL antibody. NEFL present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NEFL Antibody is added and binds to NEFL in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NEFL antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NEFL. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4645Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.049 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Neuroscience
Background
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Neurofilament, Light Polypeptide
Regulatory Status
For Research Use Only
