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Human NEK3 (Serine, Threonine-protein Kinase Nek3) ELISA Kit

Human NEK3 (Serine, Threonine-protein Kinase Nek3) ELISA Kit

The Human (NEK3) Serine, Threonine-protein Kinase Nek3 ELISA Kit measures Serine/Threonine-Protein Kinase Nek3 in samples. The plate has been pre-coated with Human NEK3 antibody. NEK3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NEK3 Antibody is added and binds to NEK3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NEK3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NEK3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E3202Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.024 ng/mL
Detection Range 0.05-20 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Enzyme & Kinase
Background Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Serine/Threonine-Protein Kinase Nek3
Regulatory Status For Research Use Only
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