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Human NEU4 (Sialidase-4) ELISA Kit
Human NEU4 (Sialidase-4) ELISA Kit
The Human (NEU4) Sialidase-4 ELISA Kit measures Sialidase-4 in samples. The plate has been pre-coated with Human NEU4 antibody. NEU4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NEU4 Antibody is added and binds to NEU4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NEU4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NEU4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3141Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
10.17 ng/L
Detection Range
20-6000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Neuroscience
Background
Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Efficiently hydrolyzes gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3 (PubMed: 15847605, PubMed: 21521691, PubMed: 15213228). Hydrolyzes poly-alpha-(2->8)-sialylated neural cell adhesion molecule NCAM1 likely at growth cones, suppressing neurite outgrowth in hippocampal neurons (By similarity). May desialylate sialyl Lewis A and X antigens at the cell surface, down-regulating these glycan epitopes recognized by SELE/E selectin in the initiation of cell adhesion and extravasation (PubMed: 21521691). Has sialidase activity toward mucin, fetuin and sialyllactose (PubMed: 15847605). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Sialidase-4
Regulatory Status
For Research Use Only
