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Human NFAT5 (Nuclear Factor of Activated T-cells 5) ELISA Kit

Human NFAT5 (Nuclear Factor of Activated T-cells 5) ELISA Kit

The Human (NFAT5) Nuclear Factor of Activated T-cells 5 ELISA Kit measures Nuclear Factor of Activated T-Cells 5 in samples. The plate has been pre-coated with Human NFAT5 antibody. NFAT5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NFAT5 Antibody is added and binds to NFAT5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NFAT5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NFAT5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E5792Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 9.09 ng/L
Detection Range 20-4500 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Immunology
Background Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity (PubMed: 10051678). Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17 (PubMed: 22266867). Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3' (PubMed: 10377394). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Nuclear Factor of Activated T-Cells 5
Regulatory Status For Research Use Only
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