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Human NKX2-5 (Homeobox Protein Nkx-2.5) ELISA Kit

Human NKX2-5 (Homeobox Protein Nkx-2.5) ELISA Kit

The Human (NKX2-5) Homeobox Protein Nkx-2.5 ELISA Kit measures Homeobox Protein Nkx-2.5 in samples. The plate has been pre-coated with Human NKX2-5 antibody. NKX2-5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NKX2-5 Antibody is added and binds to NKX2-5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NKX2-5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NKX2-5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E5433Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 8.01 ng/L
Detection Range 15-3000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Transcription factor required for the development of the heart and the spleen (PubMed: 22560297). During heart development, acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed: 22849347, PubMed: 26926761). Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression (PubMed: 22560297). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Homeobox Protein Nkx-2.5
Regulatory Status For Research Use Only
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