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Human NOX5 (Nadphoxidase 5) ELISA Kit

Human NOX5 (Nadphoxidase 5) ELISA Kit

The Human (NOX5) Nadphoxidase 5 ELISA Kit measures Nadph Oxidase 5 in samples. The plate has been pre-coated with Human NOX5 antibody. NOX5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NOX5 Antibody is added and binds to NOX5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NOX5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NOX5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E5738Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 7.34 ng/L
Detection Range 15-3000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cell Biology
Background Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton Channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Nadph Oxidase 5
Regulatory Status For Research Use Only
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