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Human NPHS1 (Nephrin) ELISA Kit
Human NPHS1 (Nephrin) ELISA Kit
The Human (NPHS1) Nephrin ELISA Kit measures Nephrin in samples. The plate has been pre-coated with Human Nphs1 antibody. Nphs1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human Nphs1 Antibody is added and binds to Nphs1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Nphs1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human Nphs1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1091Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.04 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction
Background
Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Nephrin
Regulatory Status
For Research Use Only
