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Human NSMCE2 (E3 Sumo-protein Ligase Nse2) ELISA Kit
Human NSMCE2 (E3 Sumo-protein Ligase Nse2) ELISA Kit
The Human (NSMCE2) E3 Sumo-protein Ligase Nse2 ELISA Kit measures E3 SUMO-protein ligase NSE2 in samples. The plate has been pre-coated with Human NSMCE2 antibody. NSMCE2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NSMCE2 Antibody is added and binds to NSMCE2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NSMCE2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human NSMCE2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3970Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
10.12 ng/L
Detection Range
20-3800 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling, Immunology
Background
E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination (PubMed:16055714, PubMed:16810316).
Is not be required for the stability of the complex (PubMed:16055714, PubMed:16810316).
The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks (PubMed:16055714, PubMed:16810316).
The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PubMed:17589526).
Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TSNAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, RAD51AP1, and maybe the cohesin components RAD21 and STAG2 (PubMed:16055714, PubMed:16810316, PubMed:17589526, PubMed:31400850).
Required for recruitment of telomeres to PML nuclear bodies (PubMed:17589526).
SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines (PubMed:17589526).
Required for sister chromatid cohesion during prometaphase and mitotic progression (PubMed:19502785). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
E3 SUMO-protein ligase NSE2
Regulatory Status
For Research Use Only
