The Human (OSBPL3) Oxysterol-binding Protein-related Protein 3 ELISA Kit measures Oxysterol-Binding Protein-Related Protein 3 in samples. The plate has been pre-coated with Human OSBPL3 antibody. OSBPL3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human OSBPL3 Antibody is added and binds to OSBPL3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated OSBPL3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human OSBPL3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPhosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes (PubMed: 16143324). Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments (PubMed: 25447204). The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed: 18270267, PubMed: 25447204). With VAPA, may regulate ER morphology (PubMed: 16143324). Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion (PubMed: 18270267). Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3 (PubMed: 16143324). Also binds 25-hydroxycholesterol and cholesterol (PubMed: 17428193). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteOxysterol-Binding Protein-Related Protein 3
