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Human Pancreatic Triacylglycerol Lipase (PNLIP) ELISA Kit
Human Pancreatic Triacylglycerol Lipase (PNLIP) ELISA Kit
This ELISA kit is designed to detect Human Pancreatic Triacylglycerol Lipase (Human PNLIP). The assay plate has been pre-coated with mouse anti-Human PNLIP monoclonal antibody. When the sample containing PNLIP is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human PNLIP Antibody is added to the wells and binds to PNLIP in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human PNLIP present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE274
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
4.27 pg/mL
Detection Range
15.63-1000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Cancer, Cardiovascular, Metabolism, Signal Transduction
Background
PNLIP is an enzyme that belongs to the lipase family. Secreted from the pancreas, PNLIP is the primary lipase that hydrolyzes dietary fat molecules in the human digestive system, converting triglyceride substrates found in ingested oils to monoglycerides and free fatty acids. Bile salts secreted from the liver and stored in gallbladder are released into the duodenum where they coat and emulsify large fat droplets into smaller droplets, thus increasing the overall surface area of the fat, which allows the lipase to break apart the fat more effectively. The resulting monomers (2 free fatty acids and one 2-monoacylglycerol) are then moved by way of peristalsis along the small intestine to be absorbed into the lymphatic system by a specialized vessel called a lacteal.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Pancreatic triacylglycerol lipase
Regulatory Status
For Research Use Only
