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Human PFKFB1 (6-phosphofructo-2-kinase) ELISA Kit
Human PFKFB1 (6-phosphofructo-2-kinase) ELISA Kit
The Human (PFKFB1) 6-phosphofructo-2-kinase ELISA Kit measures 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 in samples. The plate has been pre-coated with Human PFKFB1 antibody. PFKFB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PFKFB1 Antibody is added and binds to PFKFB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PFKFB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PFKFB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3190Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.022 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction, Enzyme & Kinase
Background
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 is an enzyme that in humans is encoded by the PFKFB1 gene. This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1
Regulatory Status
For Research Use Only
