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Human PGRN (Progranulin) ELISA Kit
Human PGRN (Progranulin) ELISA Kit
The Human (PGRN) Progranulin ELISA Kit measures Progranulin in samples. The plate has been pre-coated with Human GRN antibody. GRN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GRN Antibody is added and binds to GRN in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GRN antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human GRN. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1755Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
5.12 ng/mL
Detection Range
10-700 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Neuroscience
Background
Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed: 28541286, PubMed: 28073925, PubMed: 18378771, PubMed: 28453791, PubMed: 12526812). Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (PubMed: 28453791, PubMed: 28541286). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed: 28073925). In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (By similarity). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed: 12526812). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed: 18378771). [Granulin-4]: Promotes proliferation of the epithelial cell line A431 in culture. [Granulin-3]: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8. [Granulin-7]: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Progranulin
Regulatory Status
For Research Use Only
