Human PIGF (Phosphatidylinositol-glycan Biosynthesis Class F Protein) ELISA Kit
Human PIGF (Phosphatidylinositol-glycan Biosynthesis Class F Protein) ELISA Kit
The Human (PIGF) Phosphatidylinositol-glycan Biosynthesis Class F Protein ELISA Kit measures Phosphatidylinositol-Glycan Biosynthesis Class F Protein in samples. The plate has been pre-coated with Human PIGF antibody. PIGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PIGF Antibody is added and binds to PIGF in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PIGF antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PIGF. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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