The Human (PIM1) Proto-oncogene Serine Threonine-protein Kinase Pim-1 ELISA Kit measures Proto-oncogene serine/threonine-protein kinase pim-1 in samples. The plate has been pre-coated with Human PIM1 antibody. PIM1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PIM1 Antibody is added and binds to PIM1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PIM1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PIM1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasSignal Transduction, Enzyme & Kinase
BackgroundProto-oncogene serine/threonine-protein kinase Pim-1 is an enzyme that in humans is encoded by the PIM1 gene. Pim-1 is a proto-oncogene which encodes for the serine/threonine kinase of the same name. The pim-1 oncogene was first described in relation to murine T-cell lymphomas, as it was the locus most frequently activated by the Moloney murine leukemia virus. Subsequently, the oncogene has been implicated in multiple human cancers, including prostate cancer, acute myeloid leukemia and other hematopoietic malignancies. Primarily expressed in spleen, thymus, bone marrow, prostate, oral epithelial, hippocampus and fetal liver cells, Pim-1 has also been found to be highly expressed in cell cultures isolated from human tumors. Pim-1 is mainly involved in cell cycle progression, apoptosis and transcriptional activation, as well as more general signal transduction pathways. Pim-1's role in oncogenic signalling has led to it becoming a widely studied target in cancer research, with numerous drug candidates under investigation which target it. Source: UniProt Consortium (2025)
