The Human (PIPK2) Receptor-interacting Serine/Threonine-protein Kinase 2 ELISA Kit measures Receptor-Interacting Serine,Threonine-Protein Kinase 2 in samples. The plate has been pre-coated with Human RIPK2 antibody. RIPK2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RIPK2 Antibody is added and binds to RIPK2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RIPK2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human RIPK2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundSerine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Plays a role in the inactivation of RHOA in response to NGFR signaling (PubMed: 26646181). Source: UniProt Consortium (2025)
