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Human PIPK3 (Receptor-interacting Serine-Threonine Kinase 3) ELISA Kit
Human PIPK3 (Receptor-interacting Serine-Threonine Kinase 3) ELISA Kit
The Human (PIPK3) Receptor-interacting Serine-Threonine Kinase 3 ELISA Kit measures Receptor-Interacting Serine-Threonine Kinase 3 in samples. The plate has been pre-coated with Human RIPK3 antibody. RIPK3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RIPK3 Antibody is added and binds to RIPK3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RIPK3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human RIPK3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4492Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
4.59 ng/L
Detection Range
10-2000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology, Enzyme & Kinase
Background
Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death (PubMed: 19524512, PubMed: 19524513, PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 29883609). Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1 (PubMed: 19524512, PubMed: 19524513, PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 29883609, PubMed: 32298652). Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed: 19524512, PubMed: 19524513, PubMed: 22265413, PubMed: 22265414, PubMed: 22421439, PubMed: 25316792, PubMed: 29883609). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (By similarity). Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity (By similarity). Phosphorylates RIPK1: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation (PubMed: 19524513). In some cell types, also able to restrict viral replication by promoting cell death-independent responses (By similarity). In response to Zika virus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with ZBP1, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate (By similarity). Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (By similarity). RIPK3 binds to and enhances the activity of three metabolic enzymes: GLUL, GLUD1, and PYGL (PubMed: 19$498.00|$360.00109). These metabolic enzymes may eventually stimulate the tricarboxylic acid cycle and oxidative phosphorylation, which could result in enhanced ROS production (PubMed: 19$498.00|$360.00109). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Receptor-Interacting Serine-Threonine Kinase 3
Regulatory Status
For Research Use Only
