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Human PLA2G2F (Group Iif Secretory Phospholipase A2) ELISA Kit
Human PLA2G2F (Group Iif Secretory Phospholipase A2) ELISA Kit
The Human (PLA2G2F) Group Iif Secretory Phospholipase A2 ELISA Kit measures Group IIF Secretory Phospholipase A2 in samples. The plate has been pre-coated with Human PLA2G2F antibody. PLA2G2F present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PLA2G2F Antibody is added and binds to PLA2G2F in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLA2G2F antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PLA2G2F. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5362Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.049 ng/mL
Detection Range
0.1-40 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Metabolism
Background
Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at the sn-2 position of phospholipids (phospholipase A2 activity), the catalytic efficiency decreasing in the following order: phosphatidylglycerols > phosphatidylethanolamines > phosphatidylcholines > phosphatidylserines (PubMed: 11112443). May play a role in lipid mediator production in inflammatory conditions, by providing arachidonic acid to downstream cyclooxygenases and lipoxygenases (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Group IIF Secretory Phospholipase A2
Regulatory Status
For Research Use Only
