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Human PLB1 (Phospholipase B1) ELISA Kit
Human PLB1 (Phospholipase B1) ELISA Kit
The Human (PLB1) Phospholipase B1 ELISA Kit measures Phospholipase B1 in samples. The plate has been pre-coated with Human PLB1 antibody. PLB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PLB1 Antibody is added and binds to PLB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PLB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E5872Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
9.25 ng/L
Detection Range
20-4500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cell Biology, Epigenetics and Nuclear Signaling, Cancer
Background
Calcium-independent membrane-associated phospholipase that catalyzes complete diacylation of phospholipids by hydrolyzing both sn-1 and sn-2 fatty acyl chains attached to the glycerol backbone (phospholipase B activity) (By similarity).
Has dual phospholipase and lysophospholipase activities toward diacylphospholipids. Preferentially cleaves sn-2 ester bonds over sn-1 bonds. Acts as a lipase toward glycerolipid substrates (By similarity).
Hydrolyzes fatty acyl chains of diacylglycerols with preference for the sn-2 position and of triacylglycerols with not positional selectivity (By similarity).
May also hydrolyze long chain retinyl esters such as retinyl palmitate (By similarity).
May contribute to digestion of dietary phospholipids, glycerolipids and retinoids, facilitating lipid absorption at the brush border (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Phospholipase B1
Regulatory Status
For Research Use Only
