Human PLCB3 (1-Phosphatidylinositol-4, 5-bisphosphate Phosphodiesterase Beta-3) ELISA Kit
Human PLCB3 (1-Phosphatidylinositol-4, 5-bisphosphate Phosphodiesterase Beta-3) ELISA Kit
The Human (PLCB3) 1-Phosphatidylinositol-4, 5-bisphosphate Phosphodiesterase Beta-3 ELISA Kit measures 1-Phosphatidylinositol-4,5-Bisphosphate Phosphodiesterase Beta-3 in samples. The plate has been pre-coated with Human PLCB3 antibody. PLCB3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PLCB3 Antibody is added and binds to PLCB3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PLCB3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PLCB3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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