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Human PPM1D (Protein Phosphatase, Mg2+, Mn2+ Dependent 1D) ELISA Kit
Human PPM1D (Protein Phosphatase, Mg2+, Mn2+ Dependent 1D) ELISA Kit
The Human (PPM1D) Protein Phosphatase, Mg2+, Mn2+ Dependent 1D ELISA Kit measures Protein Phosphatase, Mg2+/Mn2+ Dependent 1D in samples. The plate has been pre-coated with Human PPM1D antibody. PPM1D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PPM1D Antibody is added and binds to PPM1D in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PPM1D antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PPM1D. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E7328Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.019 ng/mL
Detection Range
0.05-15 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction, Enzyme & Kinase
Background
Involved in the negative regulation of p53 expression (PubMed: 23242139). Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins (PubMed: 15870257, PubMed: 16311512). Mediates MAPK14 dephosphorylation and inactivation (PubMed: 21283629). Is also an important regulator of global heterochromatin silencing and critical in maintaining genome integrity (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Protein Phosphatase, Mg2+/Mn2+ Dependent 1D
Regulatory Status
For Research Use Only
