The Human (PPP1R8) Nuclear Inhibitor of Protein Phosphatase 1 ELISA Kit measures Nuclear Inhibitor of Protein Phosphatase 1 in samples. The plate has been pre-coated with Human PPP1R8 antibody. PPP1R8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PPP1R8 Antibody is added and binds to PPP1R8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PPP1R8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PPP1R8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundInhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing. Source: UniProt Consortium (2025)
