The Human (PSMD4) 26S Proteasome Non-atpase Regulatory Subunit 4 ELISA Kit measures 26S Proteasome Non-Atpase Regulatory Subunit 4 in samples. The plate has been pre-coated with Human PSMD4 antibody. PSMD4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PSMD4 Antibody is added and binds to PSMD4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PSMD4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PSMD4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundComponent of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains. Source: UniProt Consortium (2025)
