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Human PT (Prothrombin) ELISA Kit
Human PT (Prothrombin) ELISA Kit
The Human (PT) Prothrombin ELISA Kit measures Prothrombin in samples. The plate has been pre-coated with Human F2 antibody. F2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human F2 Antibody is added and binds to F2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated F2 antibody. After incubation unbound Streptavidin- HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human F2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E1146Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
2.49 U/L
Detection Range
5-2000 U/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cardiovascular
Background
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Prothrombin
Regulatory Status
For Research Use Only
