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Human PTGR1 (Prostaglandin Reductase 1) ELISA Kit

Human PTGR1 (Prostaglandin Reductase 1) ELISA Kit

The Human (PTGR1) Prostaglandin Reductase 1 ELISA Kit measures Prostaglandin Reductase 1 in samples. The plate has been pre-coated with Human PTGR1 antibody. PTGR1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PTGR1 Antibody is added and binds to PTGR1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PTGR1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human PTGR1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E1816Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 0.096 ng/mL
Detection Range 0.2-60 ng/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Immunology
Background NAD(P)H-dependent oxidoreductase involved in metabolic inactivation of pro- and anti-inflammatory eicosanoids: prostaglandins (PG), leukotrienes (LT) and lipoxins (LX) (PubMed: 25619643). Catalyzes with high efficiency the reduction of the 13,14 double bond of 15-oxoPGs, including 15-oxo-PGE1, 15-oxo-PGE2, 15-oxo-PGF1-alpha and 15-oxo-PGF2-alpha (PubMed: 25619643). Catalyzes with lower efficiency the oxidation of the hydroxyl group at C12 of LTB4 and its derivatives, converting them into biologically less active 12-oxo-LTB4 metabolites (PubMed: 25619643) (By similarity). Reduces 15-oxo-LXA4 to 13,14 dihydro-15-oxo-LXA4, enhancing neutrophil recruitment at the inflammatory site (By similarity). May play a role in metabolic detoxification of alkenals and ketones. Reduces alpha,beta-unsaturated alkenals and ketones, particularly those with medium-chain length, showing highest affinity toward (2E)-decenal and (3E)-3-nonen-2-one (PubMed: 25619643). May inactivate 4-hydroxy-2-nonenal, a cytotoxic lipid constituent of oxidized low-density lipoprotein particles (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Prostaglandin Reductase 1
Regulatory Status For Research Use Only
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