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Human RAPGEF4 (Rap Gguanine Nucleotide Exchange Factor 4) ELISA Kit

Human RAPGEF4 (Rap Gguanine Nucleotide Exchange Factor 4) ELISA Kit

The Human (RAPGEF4) Rap Gguanine Nucleotide Exchange Factor 4 ELISA Kit measures Rap guanine nucleotide exchange factor 4 in samples. The plate has been pre-coated with Human RAPGEF4 antibody. RAPGEF4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RAPGEF4 Antibody is added and binds to RAPGEF4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RAPGEF4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human RAPGEF4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E6056Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 7.67 ng/L
Detection Range 15-3000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction
Background Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Rap guanine nucleotide exchange factor 4
Regulatory Status For Research Use Only
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