The Human (RASSF1) Ras Association Domain-containing Protein 1 ELISA Kit measures Ras Association Domain-Containing Protein 1 in samples. The plate has been pre-coated with Human RASSF1 antibody. RASSF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RASSF1 Antibody is added and binds to RASSF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated RASSF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human RASSF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundPotential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteRas Association Domain-Containing Protein 1
