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Human Retinol-Binding Protein 4 (RBP4) ELISA Kit
Human Retinol-Binding Protein 4 (RBP4) ELISA Kit
This ELISA kit is designed to detect Human Retinol-Binding Protein 4 (Human RBP4). The assay plate has been pre-coated with mouse anti-Human RBP4 monoclonal antibody. When the sample containing RBP4 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human RBP4 Antibody is added to the wells and binds to RBP4 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human RBP4 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE070
Regular price
$624.00 USD
Regular price
$480.00 USD
Sale price
$624.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
4.31 pg/mL
Detection Range
23.44-1500 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Cardiovascular, Metabolism, Signal Transduction, Developmental Biology
Background
Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM).
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Retinol-binding protein 4
Regulatory Status
For Research Use Only
