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Human ROCK1 (Rho-associated Protein Kinase 1) ELISA Kit
Human ROCK1 (Rho-associated Protein Kinase 1) ELISA Kit
The Human (ROCK1) Rho-associated Protein Kinase 1 ELISA Kit measures Rho-Associated Protein Kinase 1 in samples. The plate has been pre-coated with Human ROCK1 antibody. ROCK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ROCK1 Antibody is added and binds to ROCK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ROCK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ROCK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3773Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.022 ng/mL
Detection Range
0.05-20 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Enzyme & Kinase
Background
Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity (PubMed: 10436159, PubMed: 10652353, PubMed: 11018042, PubMed: 11283607, PubMed: 17158456, PubMed: 18573880, PubMed: 19131646, PubMed: 8617235, PubMed: 9722579). Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A (PubMed: 10436159, PubMed: 10652353, PubMed: 11018042, PubMed: 11283607, PubMed: 17158456, PubMed: 18573880, PubMed: 19131646, PubMed: 8617235, PubMed: 9722579, PubMed: 23093407, PubMed: 23355470). Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing (PubMed: 18694941). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (PubMed: 19036714). Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation (PubMed: 19181962). Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation (PubMed: 21072057). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Promotes keratinocyte terminal differentiation (PubMed: 19997641). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Rho-Associated Protein Kinase 1
Regulatory Status
For Research Use Only
