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Human Sialic Acid-Binding Ig-Like Lectin 5 (SIGLEC5) ELISA Kit
Human Sialic Acid-Binding Ig-Like Lectin 5 (SIGLEC5) ELISA Kit
This ELISA kit is designed to detect Human Sialic Acid-Binding Ig-Like Lectin 5 (Human SIGLEC5). The assay plate has been pre-coated with mouse anti-Human SIGLEC5 monoclonal antibody. When the sample containing SIGLEC5 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human SIGLEC5 Antibody is added to the wells and binds to SIGLEC5 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human SIGLEC5 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE227
Regular price
$624.00 USD
Regular price
$480.00 USD
Sale price
$624.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
108.01 pg/mL
Detection Range
234.38-15000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Immunology
Background
SIGLEC5 contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. It belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. SIGLEC5 is expressed by monocytic/myeloid lineage cells. It is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. It is also expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Sialic acid-binding Ig-like lectin 5
Regulatory Status
For Research Use Only
