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Human Slam Family Member 5 (CD84) ELISA Kit

Human Slam Family Member 5 (CD84) ELISA Kit

This ELISA kit is designed to detect Human Slam Family Member 5 (Human CD84). The assay plate has been pre-coated with mouse anti-Human CD84 monoclonal antibody. When the sample containing CD84 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human CD84 Antibody is added to the wells and binds to CD84 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human CD84 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE018
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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Product Details

Species Reactivity Human
Sensitivity 41.67 pg/mL
Detection Range 156.25-10000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Immunology, Stem cells
Background The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein) and EAT-2 (EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. The CD84-CD84 interaction was independent of its cytoplasmic tail. Thus, CD84 is its own ligand and acts as a costimulatory molecule. CD84 is expressed on cells from almost all hematopoietic lineages and on CD34+ hematopoietic progenitor cells, suggesting that CD84 serves as a marker for committed hematopoietic progenitor cells.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte SLAM family member 5
Regulatory Status For Research Use Only
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