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Human Trna (Guanine-N(7)-)-Methyltransferase (METTL1) ELISA Kit
Human Trna (Guanine-N(7)-)-Methyltransferase (METTL1) ELISA Kit
This ELISA kit is designed to detect Human Trna (Human Guanine-N(Human 7)-)-Methyltransferase (Human METTL1). The assay plate has been pre-coated with mouse anti-Human METTL1 monoclonal antibody. When the sample containing METTL1 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human METTL1 Antibody is added to the wells and binds to METTL1 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human METTL1 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE200
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
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per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
52.43 pg/mL
Detection Range
156.25-10000 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Epigenetics and Nuclear Signaling
Background
tRNA (guanine-N(7)-)-methyltransferase, also known as Methyltransferase-like protein 1, tRNA (m7G46)-methyltransferase and METTL1, is a nucleus protein that belongs to the methyltransferase superfamily and TrmB family. METTL1 gene has been identified by its sequence similarity to the yeast ORF YDL21w. The human cDNA and the genomic structure of METTL1 have been analyzed. The transcript contains 1292 nucleotides and codes for a protein of 276 amino acids. The METTL1 gene product shows high sequence similarities to putative proteins from mouse, Drosophila melanogaster, Arabidopsis thaliana, Caenorhabditis elegans, and yeast (39.8% identity between all six species). Computer analyses of the deduced protein sequence reveal two highly conserved amino acid motifs, one of which is typical for methyltransferases. Both motifs are also present in hypothetical proteins from eubacteria. Disruption of the homologous yeast ORF YDL21w shows that the gene is at least not essential for vegetative growth in Saccharomyces cerevisiae.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
tRNA (guanine-N(7)-)-methyltransferase
Regulatory Status
For Research Use Only
