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Human Vesicular Integral-Membrane Protein Vip36 (LMAN2) ELISA Kit

Human Vesicular Integral-Membrane Protein Vip36 (LMAN2) ELISA Kit

This ELISA kit is designed to detect Human Vesicular Integral-Membrane Protein Vip36 (Human LMAN2). The assay plate has been pre-coated with mouse anti-Human LMAN2 monoclonal antibody. When the sample containing LMAN2 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human LMAN2 Antibody is added to the wells and binds to LMAN2 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human LMAN2 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.

Catalog No: BPE252
Regular price $754.00 USD
Regular price $580.00 USD Sale price $754.00 USD
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Product Details

Species Reactivity Human
Sensitivity 11.45 pg/mL
Detection Range 31.25-2000 pg/mL
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 3.5 hour(s)
Research Areas Signal Transduction
Background LMAN2 (Lectin, Mannose Binding 2, also known as VIP36) is a Protein Coding gene. This gene encodes a type I transmembrane lectin that shuttles between the endoplasmic reticulum, the Golgi apparatus, and the plasma membrane. The encoded protein binds high mannose type glycoproteins and may facilitate their sorting, trafficking, and quality control. The L-type lectin LMAN2 appears to be specifically required for the accumulation of GPRC5B in the Golgi complex and restriction of GPRC5B transport along the exosomal pathway. This may occur due to interference with the adaptor protein GGA1-mediated trans-Golgi network-to-endosome transport of GPRC5B. A Golgi-traversing pathway for the exosomal release of the cargo protein GPRC5B in which CD2AP facilitates the entry and LMAN2 impedes the exit of the flux, respectively.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life This product can be stored at 2-8C.
Analyte Vesicular integral-membrane protein VIP36
Regulatory Status For Research Use Only
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