The Matrix metalloproteinase 3 (MMP-3) ELISA is an enzyme immunoassay for the quantitative determination of Matrix metalloproteinase 3 (MMP-3) in human serum and plasma.
The microtiter plate is coated with the antibody specifically binding the Matrix metalloproteinase 3. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of MMP-3 in the specimen. The concentration of MMP-3 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.
Catalog No:BA1013
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Product Details
Species ReactivityHuman
Sensitivity0.05 ng/mL
Detection Range0.2 - 10 ng/mL
Sample TypeSerum, Plasma
Sample Size10 uL
Incubation(s)2.5 hour(s)
Research AreasPulmonary
BackgroundMatrix metalloproteinases (MMPs) are zinc-dependent endopeptidases, responsible for the integrity of the basement membrane (BM) via degradation of extracellular matrix and BM components. MMPs are presented in various types of cells, including cardiomyocytes, cerebral neurons, hepatocytes, and many others, thus influencing various processes.
MMP-3, also called stromelysin 1, is reported to be found in the cytosol of dopaminergic cells where its activation under stress conditions contributes to neuronal degradation. In chondrocytes, nuclear MMP-3 is suggested to be involved in the development of inflammatory arthritic diseases. Matrix metalloproteinase-3 (MMP-3) is implicated in the formation of atherosclerotic plaques, and the MMP-3 -1612 5A/6A polymorphism is associated with myocardial infarction (MI) and stable coronary artery disease (CAD).
