Mouse Akr1c6 (Estradiol 17 Beta-Dehydrogenase 5) ELISA Kit
Mouse Akr1c6 (Estradiol 17 Beta-Dehydrogenase 5) ELISA Kit
This Estradiol 17 Beta-Dehydrogenase 5 ELISA kit is based on Sandwich ELISA detection method and takes approximately 4 hours. The microplate provided in this kit has been precoated with anti Akr1c6 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with Akr1c6 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of Akr1c6 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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