• FALSE
1 1

Mouse NF-kB-p65 (Nuclear Factor Kappa B p65) ELISA Kit

Mouse NF-kB-p65 (Nuclear Factor Kappa B p65) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NF-kB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NF-kB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NF-kB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NF-kB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

Catalog No: ELK9154
$764.00 USD
$588.00 USD $764.00 USD
Sale Sold out
Packaging
2 weeks lead time
Download PDF

Product Details

Species Reactivity Mouse
Sensitivity 12.2 pg/mL
Detection Range 31.25-2000 pg/mL
Sample Type serum, plasma, tissue homogenates
Incubation(s) 3.5h
Research Areas Immunology
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life If the kit is not opened, store the whole kit: 4C(short time storage, valid for 6 months); -20C(long-term storage, valid for 1 year). Avoid repeated freeze-thaw cycles If the kit is opened, Store the whole kit at 4C. If the kit is not used up in 1 week. Store the Pre-Coated Microplate, Standard ,Biotinylated-Conjugate and Streptavidin-HRP at -20C, the rest reagents at 4C, please used up within 6 months.
Analyte NF-kB Kits
Regulatory Status For Research Use Only
View full details

Inquire about this product