• Osteopontin (OPN) ELISA
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Osteopontin (OPN) ELISA

Osteopontin (OPN) ELISA

The Osteopontin ELISA is an enzyme immunoassay for the quantitative determination of Osteopontin in human serum and plasma.

The microtiter plate is coated with the antibody specifically binding the Osteopontin. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of Osteopontin in the specimen. The concentration of Osteopontin in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.

Catalog No: BA1028
$560.00 USD
$560.00 USD $560.00 USD
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2 weeks

Product Details

Species Reactivity Human
Sensitivity 0.78 ng/mL
Detection Range 125 - 4000 pg/mL
Sample Type Serum, Plasma
Sample Size 6 uL
Incubation(s) 4.5 hour(s)
Research Areas Autoimmunity
Background Osteopontin (OPN), a secreted multifunctional phosphoprotein, is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family of cell matrix proteins and participates in many biological activities. OPN has been demonstrated to be closely related to the occurrence and development of many bone-related diseases, such as osteoporosis, rheumatoid arthritis, and osteosarcoma. Besides that, it functions as a proinflammatory cytokine and promotes cell-mediated immune responses, and also has protective functions such as biomineralization and wound healing, and is also a strong predictor of adverse outcomes in patients with CVDs. Thus, OPN is not only a risk factor but also a potential therapeutic target for CVDs.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Osteopontin
Regulatory Status For Research Use Only
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