Rat ADH1 (Alcohol Dehydrogenase 1) ELISA Kit
Rat ADH1 (Alcohol Dehydrogenase 1) ELISA Kit
This Alcohol Dehydrogenase 1 ELISA kit is based on Sandwich ELISA detection method and takes approximately 4 hours. The microplate provided in this kit has been precoated with anti ADH1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with ADH1 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of ADH1 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
Couldn't load pickup availability
Product Details
