The Rat Clusterin ELISA is an enzyme immunoassay for the quantitative determination of Clusterin in rat serum and rat urine.
In the Rat Clusterin ELISA kit, standards and samples are incubated in microtitration wells pre-coated with polyclonal anti-rat clusterin antibody. After 60 minutes incubation followed by washing, biotin-labelled polyclonal anti-rat clusterin antibody is added and incubated with the captured clusterin for 60 minutes. After another washing, streptavidin-HRP conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of rat Ccusterin. A standard curve is constructed by plotting absorbance values against clusterin concentrations of standards and concentrations of unknown samples are determined using this standard curve.
Catalog No:BA2011
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Product Details
Species ReactivityRat
Sensitivity0.5 ng/mL
Detection Range4 - 128 ng/mL
Sample TypeSerum, Plasma, Urine
Sample Size5 to 10 uL
Incubation(s)2.5 hour(s)
Research AreasNephrology
BackgroundClusterin (Apolippoprotein J; SP-40,40; TRPM-2; SGP-2; pADHC-9; CLJ; T64; GP III; XIP8) is a highly conserved disulfide-linked secreted heterodimeric glycoprotein of 75-80 kDa but truncated forms targeted to nucleus have also been identified.
Clusterin is highly conserved across species, showing 70-80% identity at the amino acid level amongst mammals, and numerous variants and isoforms have been described. The protein is constitutively secreted by a number of cell types including epithelial and neuronal cells and is a major protein in physiological fluids including plasma, milk, urine, cerebrospinal fluid and semen. Due to its wide tissue distribution many diverse physiological functions have been attributed to clusterin including sperm maturation, membrane recycling, lipid transportation, tissue remodeling, complement inhibition and cell-cell or cell-substratum interactions. Moreover, it was proposed, that clusterin functions is as an extra cellular chaperon that stabilizes stressed proteins in a folding-competent state and protein has also been implicated in programmed cell death. Another defining prominent of clusterin is its induction in many severe physiological disturbances states including kidney degenerative diseases, prostate and vesicle carcinogenesis, ovarian cancer and several neurodegenerative conditions.
Interesting study determine that urinary clusterin levels in the rat correlate with severity of tubular damage and may help to differentiate between glomerular and tubular injuries.
